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一步法人超敏C反应蛋白(Hs-CRP)酶联免疫吸附检测试剂盒

ELK037ES

规格：	价格：
48T	￥1960.00
96T	￥2800.00

下载说明 ①

Overview

Product name:	EasyStep Human hs-CRP(high sensitivity C Reactive Protein) ELISA Kit
Reactivity:	Human
Alternative Names:	C-RP; PTX1; Pentraxin-Related; Pentraxin 1
Assay Type:	Sandwich
Sensitivity:	21 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.13-5000 pg/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;
Uniprot ID:	P02741
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CRP, and the Human CRP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human CRP. After TMB substrate solution is added, only those wells that contain Human CRP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CRP in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
5000.00	2.185	2.096
2500.00	1.527	1.438
1250.00	1.111	1.022
625.00	0.813	0.724
312.50	0.591	0.502
156.25	0.239	0.150
78.13	0.187	0.098
0.00	0.089	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CRP and the recovery rates were calculated by comparing the measured value to the expected amount of CRP in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	81-95%	88%
Heparin plasma(n=5)	80-95%	87%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CRP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.