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One Step SYBR Green RT-PCR Mix
EQ007
规格: 价格:
20µl x 100rxn ¥980.00
20µl x 500rxn ¥3800.00

Overview 文献

Product name: One Step SYBR Green RT-PCR Mix
产品介绍: This product is a special reagent for Real Time One Step RT-PCR using the probe method. Using this product for Real Time RT-PCR reaction can continuously perform reverse transcription and PCR amplification in the same reaction tube. It is simple to operate and can effectively prevent contamination. Since this reaction system can monitor the amplified products in real time, the detection sensitivity is greatly improved, and the electrophoresis step after PCR reaction is omitted, which is very suitable for the detection of RNA viruses.This product uses high-efficiency reverse transcriptase and high-specificity hot-start Taq DNA polymerase to perform stable and efficient Real Time One Step RT-PCR reactions. For the fluorescent quantitative PCR instrument that uses ROX as the calibration dye, this product is equipped with a separate ROX dye to correct the fluorescent signal error generated between the wells of the quantitative PCR instrument.
产品特点: 1.只需加入RNA,即可在同一PCR管内连续进行逆转录和PCR扩增,操作非常简便。

2.对扩增产物进行实时检测,大大提高了检测灵敏度,并能有效防止污染。

3.优选的三代逆转录酶配套热启动Taq酶使用以获得最佳的扩增效果,有效降低了非特异性扩增。

4.省略了PCR反应后的电泳步骤,非常适合于微量RNA的检测。
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